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Fast HILIC Analysis of Hydrophilic Dipeptides

Hydrophilic compounds usually show poor retention when using reversed phase (RP) columns for their separation. Fortunately, as a chromatographer one can resort to other separation techniques that allow for better retention of hydrophilic compounds. For example, when using HILIC (hydrophilic interaction chromatography) as a separation mode the elution order is completely reversed compared to RP resulting in high retention times for hydrophilic compounds which would have been eluted (too) early using RP columns...

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