Fast UHPLC analysis of commercial monoclonal antibodies at elevated temperatures using YMC-Triart Bio C4

contributed by YMC | 02/17/2020

Monoclonal antibodies (MAbs) are immunologically active proteins, which bind specifically to certain cells or proteins. This stimulates the immune system to attack those targets. The importance of MAbs is becoming increasingly important for the treatment of different types of cancer and autoimmune diseases. Nowadays, a wide variety of therapeutic antibodies are available on the market and several more are in research and development.

Intact antibodies are usually analysed by IEX, SEC or HIC because of their high molecular weight (about 150 kDa). Mass spectrometry (MS) compatible RP methods have also become a useful tool. In the past, a lack of sensitivity and resolution has been a hurdle. With modern RP phases addressing the requirements of these analytes, it is now easier to find a suitable method.

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE!

Email*

Choose a password*

I have read and understand the Privacy Notice *

Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

Receive communication from The Cannabis Scientist I want to stay up to date with the Mass Spectrometry field I want to stay up to date with the Spectroscopy field

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.