Monoclonal antibodies (MAb) are immunologically active proteins. They recognise and bind specifically to certain cells or proteins that contain the specific antigen. After the binding process the immune system is stimulated to attack those cells or proteins.
This behaviour of MAbs is applicable for the treatment of different kinds of cancer and autoimmune diseases. A broad variety of therapeutically antibodies are commercially available and several more are in research.
Antibodies are often analysed in HIC mode (hydrophobic interaction chromatography) because of the non-denaturating conditions used and the high selectivity to changes of protein surface-associated hydrophobicity.
An organic solvent in the mobile phase can lead to the denaturation of the antibodies, though with the addition of 15 % protic isopropanol the denaturation is limited and higher recoveries are achieved.
>> Download the full Application Note as PDF
Newsletters
Receive the latest analytical scientist news, personalities, education, and career development – weekly to your inbox.
