This application note demonstrates how the Empore™ C18 StageTips could
be applied to high pH peptide fractionation in global proteomics to significantly
increased peptide and protein identification.
Extensive off-line peptide fractionation is used to reduce the complexity of the proteome and is critical to in-depth global proteome profiling. However, the fractionation of protein samples in the sub-microgram range can be limited due to sample loss during the fractionation process. Thus, it is essential to develop efficient microscale fractionation strategies. High-pH peptide fractionation is one of the methods among these efforts. Previous methods have either used a pump- based approach (e.g., using off-line HPLC), which is sophisticated and expensive, or use beads-based approaches, which are unreliable (e.g., need to weigh certain amount of bead material and do packing every time). Here, a novel high-pH peptide fractionation method using Empore C18 StageTips is demonstrated. The results show the method is simple, reliable, and robust.
>> Download the Application Note as a PDF
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