High Resolution and High Efficiency Separations of mAbs and ADCs Using Proteomix HIC Columns

Introduction

A variety of products derived from monoclonal antibodies (mAbs), including mAb fragments and antibody-drug conjugates (ADCs), are being developed for the treatment of cancer and other diseases due to their increased potency combined with reduced toxicity. However, the efficacy of these molecules is highly dependent upon the target site-specificity and binding properties of the mAb, the linker stability, the potency of the drug, and both the distribution and number of drug species on the mAb.1 These requirements highlight the importance of characterizing these highly heterogeneous products using appropriate analytical techniques in order to assess and monitor them during manufacturing and subsequent storage.

Hydrophobic interaction chromatography (HIC) is a technique for protein separations and has been commonly used as an orthogonal method to size exclusion chromatography (SEC) and ion exchange (IEX) chromatography for the characterization of mAbs. Here we introduce Proteomix® HIC columns which have been designed for high resolution and high efficiency separations of proteins, oligonucleotides, and peptides.