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The Analytical Scientist / App Notes / 2022 / High Speed Analysis of Amino Acids in Food Using Automated Pretreatment

High Speed Analysis of Amino Acids in Food Using Automated Pretreatment

11/29/2022

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  • The analysis time is significantly reduced compared to the post-column derivatization method.
  • The analytical method is very simple and easy since the burdensome derivatization process can be performed automatically
  • Users can analyze amino acids with a simple HPLC system.


Although the post-column derivatization method has been commonly used for amino acid analysis with HPLC, its disadvantages include the long analysis time due to the characteristics of the column and high cost from the complex instrument configuration. On the other hand, although precolumn derivatization enables fast analysis with simple instrument configuration, its problems include the burdensome derivatization operation and the effect of the sample matrix. In Application News 01-00441-EN, we introduced a pre-column derivatization method for amino acid analysis using the automatic pretreatment function. With this feature, the burdensome derivatization process can be performed automatically. In this article, we introduce the examples of analyzing amino acids in various foods using the method with automatic function.

>> Download the Application Note as a PDF

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