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High throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients

The timsTOF Pro offers a combination of two unique technologies, namely a 4th dimension provided by Trapped Ion Mobility Spectrometry (TIMS) to enhance ion separation and sensitivity and Parallel Accumulation Serial Fragmentation (PASEF [1]) to improve ion utilization efficiency and data acquisition speed.


In this application note, we demonstrate the benefits of dia-PASEF technology on the timsTOF Pro platform coupled to an Evosep One for high-throughput in-depth proteome analysis of up to 300 samples per day (SPD). We quantify about 5200 protein groups in only 21- minutes run time. In 4.8-minutes ultra-high throughput runs (300 SPD) we are still able to quantify more than 2000 protein groups and 8500 peptides.

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