HILIC analysis of oligonucleotides using bioinert columns

contributed by YMC | 03/16/2023

Oligonucleotides have become more important for medical applications, currently they are used to treat several diseases. Therefore, robust and highly sensitive analytical methods are required. Ion pair reversed phase liquid chromatography (IP-RP) and anion exchange chromatography (AEX) seem to be the gold standards for the characterisation of oligonucleotides and their by-products. In addition to AEX, hydrophilic interaction liquid chromatography (HILIC) can provide an alternative approach due to the highly polar nature of oligonucleotides.

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