Selecting the Optimal Column for Native SEC-MS of Monoclonal Antibodies

contributed by Tosoh | 11/17/2022

Characterization of monoclonal antibodies (mAbs) is essential to product safety and efficacy. Determining the purity and characterizing the impurities such as dimers or fragments are two critical quality parameters. Size exclusion chromatography (SEC) coupled with mass spectrometry (MS) is increasingly used to identify the accurate molecular mass of mAbs. However, traditional SEC typically generates high particle shedding, which decreases ionization efficiency over time, even when operating in high molecular weight (HMW) m/z ranges. To avoid shedding for MS and multi-angle light scattering (MALS) applications, Tosoh Bioscience developed TSKgel® UP-SW3000-LS U/HPLC size-exclusion columns.

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE!

Email*

Choose a password*

I have read and understand the Privacy Notice *

Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

Receive communication from The Cannabis Scientist I want to stay up to date with the Mass Spectrometry field I want to stay up to date with the Spectroscopy field

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.