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Selecting the Optimal Column for Native SEC-MS of Monoclonal Antibodies

Characterization of monoclonal antibodies (mAbs) is essential to product safety and efficacy. Determining the purity and characterizing the impurities such as dimers or fragments are two critical quality parameters. Size exclusion chromatography (SEC) coupled with mass spectrometry (MS) is increasingly used to identify the accurate molecular mass of mAbs. However, traditional SEC typically generates high particle shedding, which decreases ionization efficiency over time, even when operating in high molecular weight (HMW) m/z ranges. To avoid shedding for MS and multi-angle light scattering (MALS) applications, Tosoh Bioscience developed TSKgel® UP-SW3000-LS U/HPLC size-exclusion columns.

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