The separation of certain small molecules can be influenced by the use of stainless steel column hardware. Metal-coordinating compounds such as chelating agents can interact with the metallic surface of the column body, which can adversely affect the analysis. The interaction can lead to loss of recovery, deterioration of peak shapes and sample carry over. The impact is enhanced when new columns are used so that tedious pre-conditioning is necessary.
The YMC-Accura column hardware with a strict bioinert coating of the column body and frits is designed to prevent unwanted interaction of the analyte with metal surfaces. In this application note the analysis of two chelating agents 8-hydroxyquinoline and hinokitiol, using the bioinert YMC-Accura Triart C18 is compared to the analysis using the corresponding conventional column with stainless steel hardware.
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