How to automate protocols for SYBR Green qPCR master mix preparation

contributed by INTEGRA Biosciences | 04/19/2023

Automating qPCR master mix preparation is often considered extremely tedious, due to the need to constantly change program set-up based on varying master mix volumes and sample numbers. However, the D-ONE single channel pipetting module for the ASSIST PLUS pipetting robot simplifies the programming of automated qPCR master mix preparation protocols down to a single step. The D-ONE individually reaches tubes of different qPCR master mix components, while the VIALAB program takes care of all calculations and provides automatic tip selection.

This solution allows master mixes to be pipetted into any tube or reservoir, enabling large-scale qPCR master mix preparation, with subsequent distribution to plates with multichannel pipettes to speed up the process. Here, we verify a SYBR® Green qPCR protocol prepared directly in a divided reservoir using the D-ONE on the ASSIST PLUS.

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE!

Email*

Choose a password

I have read and understand the Privacy Notice *

Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

Receive communication from The Cannabis Scientist I want to stay up to date with the Mass Spectrometry field I want to stay up to date with the Spectroscopy field

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.