Automating qPCR master mix preparation is often considered extremely tedious, due to the need to constantly change program set-up based on varying master mix volumes and sample numbers. However, the D-ONE single channel pipetting module for the ASSIST PLUS pipetting robot simplifies the programming of automated qPCR master mix preparation protocols down to a single step. The D-ONE individually reaches tubes of different qPCR master mix components, while the VIALAB program takes care of all calculations and provides automatic tip selection.
This solution allows master mixes to be pipetted into any tube or reservoir, enabling large-scale qPCR master mix preparation, with subsequent distribution to plates with multichannel pipettes to speed up the process. Here, we verify a SYBR® Green qPCR protocol prepared directly in a divided reservoir using the D-ONE on the ASSIST PLUS.
Key benefits:
Fully automated liquid handling of various qPCR master mix components on the ASSIST PLUS pipetting robot with the D-ONE single channel pipetting module ensures the reproducibility of results.
- qPCR master mix components and volumes can be easily adjusted in VIALAB to perform different qPCR protocols. The final calculation is automatically done by VIALAB according to the selected reaction count.
- The D-ONE's liquid level detection allows the use of reagents with different aliquot volumes, and informs the user if there is insufficient liquid. Furthermore, the automated tip selection guarantees high precision when pipetting different volumes.
- qPCR master mixes can be pipetted into various target vessels, including INTEGRA's multichannel reservoirs with a very low dead volume, to speed up the plating of master mixes using a multichannel pipette.
>> Download the Application Note as a PDF
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