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How to automate protocols for SYBR Green qPCR master mix preparation


Automating qPCR master mix preparation is often considered extremely tedious, due to the need to constantly change program set-up based on varying master mix volumes and sample numbers. However, the D-ONE single channel pipetting module for the ASSIST PLUS pipetting robot simplifies the programming of automated qPCR master mix preparation protocols down to a single step. The D-ONE individually reaches tubes of different qPCR master mix components, while the VIALAB program takes care of all calculations and provides automatic tip selection.

This solution allows master mixes to be pipetted into any tube or reservoir, enabling large-scale qPCR master mix preparation, with subsequent distribution to plates with multichannel pipettes to speed up the process. Here, we verify a SYBR® Green qPCR protocol prepared directly in a divided reservoir using the D-ONE on the ASSIST PLUS.

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