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Synthetic oligonucleotides are extremely promising candidates for biopharmaceuticals in a wide range of  diseases. This is why nucleic acid therapeutics such as silencing RNA (siRNA), messenger RNA (mRNA) and antisense oligonucleotides (ASOs) are gaining more and more attention in current research. Phosphoramidites are the essential part of the chemical synthesis of oligonu-cleotides, short fragments of nucleotides and analogues. To prevent any side reactions on residual reactive sites such as hydroxyl and amino groups during oligonucleotide synthesis, these groups need to be protected.

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