HPLC sample preparation— a study using Whatman filtration devices

Sample preparation methods for HPLC analysis have the potential to influence analytical results. Disposable devices can alter the sample in several ways. For example, they can release extractables into the sample, bind analytes nonspecifically, or retain liquid. This white paper presents experimental data for several of Cytiva’s syringe and syringeless filters evaluated on these properties. All tested devices contained membranes composed of regenerated cellulose, which has broad compatibility for aqueous and organic solvents. The results support the use of the tested devices for typical HPLC sample preparation needs. Further, the data supports Cytiva’s recommendation that users consider standardizing on a membrane such as RC across multiple encapsulated formats. Standardization reduces the variation associated with sample preparation on multiple membranes and increases lab process consistency.