Improving selectivity, resolution and purification productivity using pH

Introduction

Reversed phase chromatography based on traditional silica is the workhorse for the separation and purification of samples in the analytical, drug discovery and development laboratories. The pH range where these traditional stationary phases operate is between 2 and 8. Scientists regularly come across applications where selectivity, resolution as well as loading onto the column are challenging at the above mentioned pH range. Therefore, researchers seek an alternative stationary phase material that resists and has a consistent performance at a wider pH window to effectively and efficiently analyze challenging mixtures as well as isolate compounds outside the common pH range.

Organosilane enforced silica based materials with high mechanical and chemical stability at a wider range of pH constitute an important alternative for scientists to use in separation and purification of samples. This application note illustrates the utility of such enforced phases using pH as a tool. This work also shows that consistent packing of stable materials across different column sizes allows for straight forward scale-up.