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Improving the UHPLC separation of fatty acid methyl ester isomers by column coupling

The separation of very similar substances such as isomers has proved as challenging. (U)HPLC phases with a high steric selectivity are required for such analyses. In this application note the fatty acid methyl esters methyl linolenate and methyl γ-linolenate are separated using YMC-Triart C18 ExRS. Due to its exceptional high hydrophobicity and steric selectivity, YMC-Triart C18 ExRS is especially suitable for the analysis of these hydrophobic isomers.

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