Application details
- Analysis and quantification of vitamin D2/D3 metabolites 25-hydroxyvitamin D2/D3 in two human patient plasma and serum samples via reversed phase LC-MS/MS.
- Samples were prepared via simple protein precipitation and two internal standards 2H3-25-OH vitamin D2/D3 were utilized.
- As complete elimination of endogenous levels of 25-OH Vitamin D2/D3 in human serum/plasma is impossible, deuterated 2H6-25-OH Vitamin D2/D3 were used.
- Standard curves were created from standard samples where ratio of deuterated 25-OH vitamin D2/D3 and two internal standards 2H3-25-OH vitamin D2/D3 were plotted against ratio of concentrations of the same.
Ordering Information
- Purospher® STAR RP-18 endcapped (2μm) Hibar® HR 100-2.1 (Cat. No. 150648)
- Methanol hypergrade for LC-MS LiChrosolv® (Cat. No. 106035)
- Water for chromatography LiChrosolv® (LC-MS) (Cat. No. 115333 or Milli-Q® water from water purification systems)
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