Sample Prep Method Development and Optimization for the LC/MS/MS Analysis of Steroid Hormones in Plasma

contributed by Sigma Aldrich | 02/27/2015

HybridSPE^®-Phospholipid plates provided excellent analyte recovery of these difficult compounds from plasma samples with subsequent LC/MS/MS analysis on Ascentis^® Express Fused-Core^® C18 columns. The extracts were free of endogenous phospholipids that can interfere with quantitation, decrease sample throughput and reduce column lifetime.

There is a growing trend toward converting some clinical methods from immunoassay to LC/MS/MS for a variety of reasons. LC/MS/MS improves assay specificity, is not limited by antibody availability, and allows multiplexed analyte assays to be conducted simultaneously. However, LC/MS/MS is not without its limitations, most notably interferences from endogenous sample matrix, which can result in seemingly random and arbitrary discrimination in analyte response, among other effects.¹

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