In HPLC analysis, column and composition of mobile phase greatly affect the retention and the separation of the target compounds. Many mobile phase compositions of different pH, salt concentration and organic solvent ratio need to be confirmed to determine the optimal analytical conditions for the target compounds. Columns with different stationary phases (ODS, C8, Phenyl, etc.) should also be considered. It requires considerable labor, time, and skill. By adding a flow line switching valve to the integrated HPLC iSeries, up to six columns can be used to search for analytical conditions (method scouting) in combination with four mobile phase solvents.
The system also has a mobile phase blending function that can automatically mix up to four solvents at any desired ratio. With only a few solvents prepared in advance, the optimal analytical conditionsfor the target compounds can be quickly discovered. This article introduces a method scouting workflow of the simultaneous analysis of twelve tar dyes using i-Series method scouting system.
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