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Method Scouting for Simultaneous Analysis of Twelve Tar Dyes


In HPLC analysis, column and composition of mobile phase greatly affect the retention and the separation of the target compounds. Many mobile phase compositions of different pH, salt concentration and organic solvent ratio need to be confirmed to determine the optimal analytical conditions for the target compounds. Columns with different stationary phases (ODS, C8, Phenyl, etc.) should also be considered. It requires considerable labor, time, and skill. By adding a flow line switching valve to the integrated HPLC iSeries, up to six columns can be used to search for analytical conditions (method scouting) in combination with four mobile phase solvents.

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