Conexiant
Login
  • The Analytical Scientist
  • The Cannabis Scientist
  • The Medicine Maker
  • The Ophthalmologist
  • The Pathologist
  • The Traditional Scientist
The Analytical Scientist
  • Explore

    Explore

    • Latest
    • News & Research
    • Trends & Challenges
    • Keynote Interviews
    • Opinion & Personal Narratives
    • Product Profiles
    • App Notes

    Featured Topics

    • Mass Spectrometry
    • Chromatography
    • Spectroscopy

    Issues

    • Latest Issue
    • Archive
  • Topics

    Techniques & Tools

    • Mass Spectrometry
    • Chromatography
    • Spectroscopy
    • Microscopy
    • Sensors
    • Data and AI

    • View All Topics

    Applications & Fields

    • Clinical
    • Environmental
    • Food, Beverage & Agriculture
    • Pharma and Biopharma
    • Omics
    • Forensics
  • People & Profiles

    People & Profiles

    • Power List
    • Voices in the Community
    • Sitting Down With
    • Authors & Contributors
  • Business & Education

    Business & Education

    • Innovation
    • Business & Entrepreneurship
    • Career Pathways
  • Events
    • Live Events
    • Webinars
  • Multimedia
    • Video
Subscribe
Subscribe

False

The Analytical Scientist / App Notes / 2023 / MnESI-MS platform for LC-MS analysis of native protein complexes

MnESI-MS platform for LC-MS analysis of native protein complexes

09/11/2023

Share

Featured Image

To establish a Microflow-nanospray ESI-MS (MnESI-MS) platform for LC-native MS analysis of labile native protein complexes, that achieves high throughput, high sensitivity, and robustness, using the Newomics® MnESI source and M3 multinozzle emitters interfaced with the Thermo Scientic™ Q Exactive™ UHMR Hybrid Quadrupole-Orbitrap™ Mass Spectrometer.

Native mass spectrometry (MS) maintains a biomolecule’s natural folded state and associated non-covalent interactions for mass spectrometry analysis, and is a powerful technique for studying the structure of intact proteins, large protein complexes, and protein-protein, protein-ligand interactions [1-4]. Currently, one of the biggest challenges for native MS is the analysis of large native protein complexes and their mixtures in a high-throughput manner. The current static nanoelectrospray MS (nanoESI-MS) method requires delicate technique for sample introduction and lengthy buffer exchange process for sample preparation, suffers from low reproducibility and robustness, and has a low sample throughput [5-8]. On the other hand, the conventional analytical flow LC-MS method has not been adopted widely for native MS studies of large bioorganic complexes because it does not have the sensitivity of static nanoESI-MS or may not be able to maintain the native state of labile protein complexes during mass spectrometry analysis. To directly address the challenges, we have developed a new Microflow-nanospray ESI-native MS (MnESI-MS) platform with a MnESI source and M3 emitters interfaced with the Q Exactive UHMR mass spectrometer, to achieve high-sensitivity and high-throughput LC-MS analysis of bioorganic complexes while maintaining their native state [9-12]. We have validated our platform using GroEL, r20S, and t20S proteasome as model complexes and demonstrated its utilities for cryo-EM sample screening.

>> Download the Application Note as a PDF

Newsletters

Receive the latest pathologist news, personalities, education, and career development – weekly to your inbox.

Newsletter Signup Image

Explore More in Pathology

Dive deeper into the world of pathology. Explore the latest articles, case studies, expert insights, and groundbreaking research.

False

Advertisement

Recommended

False

Related Content

Technical note: Why choose a time- of-flight mass spectrometer?
Technical note: Why choose a time- of-flight mass spectrometer?

April 28, 2023

Why TOF MS is an ideal for delivering fast, highly...

Technical note: Tandem Ionisation® – Revolutionary soft ionisation to enhance confidence in identification
Technical note: Tandem Ionisation® – Revolutionary soft ionisation to enhance confidence in identification

April 26, 2023

Download this technical note which describes Tandem Ionisation theory and practice...

ChromSpace - A powerful and versatile software platform for multi-dimensional GC
ChromSpace - A powerful and versatile software platform for multi-dimensional GC

May 1, 2023

ChromSpace® a GC software package that provides st...

Air quality studies: Increasing productivity & data precision
Air quality studies: Increasing productivity & data precision

January 12, 2023

Studies of VOCs take several years to complete...

False

The Analytical Scientist
Subscribe

About

  • About Us
  • Work at Conexiant Europe
  • Terms and Conditions
  • Privacy Policy
  • Advertise With Us
  • Contact Us

Copyright © 2025 Texere Publishing Limited (trading as Conexiant), with registered number 08113419 whose registered office is at Booths No. 1, Booths Park, Chelford Road, Knutsford, England, WA16 8GS.