Cation exchange chromatography (CEX) is perceived to be the gold standard for the charge sensitive characterisation of monoclonal antibodies (MAbs). Acidic and basic variants caused by chemical or enzymatic modifications can be separated from the main isoform of the MAb. These antibody variants have to be critically evaluated as differences in impurities and/or degradation products can lead to severe undesirable side effects.
This application note is based on data produced by the University of Geneva, School of Pharmaceutical Sciences according to the method described by Yan et al. [1]. It shows the analysis of 28 MAbs with different isoelectric points (pI 6.1–9.4) using YMC’s BioPro IEX SF column. Each MAb could be separated from acidic and basic variants which can be further characterised if coupled to a mass spectrometer (possible setup described by Yan et al.).
To achieve an acceptable elution window the initial and final ratio of mobile phase B was tuned for each MAb depending on its isoelectric point starting with an initial ratio of 20–30%B (higher ratio for MAbs with higher pI).