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Native AEX-MS analysis of IgG4-based mAbs

Cation exchange chromatography (CEX) is an excellent method of characterising the charge heterogeneity of biomolecules. This is also true for most commercially available monoclonal antibodies (mAbs). They are often IgG1- based and possess a high isoelectric point (pI) of usually ≥8. Therefore, CEX coupled with mass spectrometry (MS) is the traditional approach [1]. In contrast, anion exchange chromatography (AEX) has only been used for relatively acidic proteins.

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