Native AEX-MS analysis of IgG4-based mAbs
contributed by YMC |
Cation exchange chromatography (CEX) is an excellent method of characterising the charge heterogeneity of biomolecules. This is also true for most commercially available monoclonal antibodies (mAbs). They are often IgG1- based and possess a high isoelectric point (pI) of usually ≥8. Therefore, CEX coupled with mass spectrometry (MS) is the traditional approach . In contrast, anion exchange chromatography (AEX) has only been used for relatively acidic proteins.
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