On-Column Digest and SPE Desalting Using Tecan’s NBE™ Columns
contributed by Tecan |
Introduction
This application note demonstrates the analysis of trypsin-digested bovine serum albumin (BSA) with on- column digestion using Tecan’s Narrow Bore Extraction (NBE) columns, followed by peptide clean-up by Solid Phase Extraction (SPE) utilizing Atlas® sorbent in the same column. Resulting recoveries were then compared to standard in-tube trypsin digestion followed by subsequent sample clean-up using the same Atlas sorbents in SPE columns.
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