PCR product purification with QIAquick® 96 PCR Purification Kit and the VIAFLO 96 handheld electronic pipette
Semi-automated PCR product purification on the VIAFLO 96 handheld electronic pipette
contributed by INTEGRA Biosciences |
QIAquick 96 PCR Purification Kit is suitable for purifying up to 10 μg of material for downstream applications, such as sequencing, cloning, labeling and microarrays. The kit facilitates the removal of impurities like primers, unincorporated nucleotides, buffers, salts, mineral oils, agarose and enzymes. The vacuum-driven process is much faster than centrifugation, and gives high, reproducible yields. It is important to avoid cross-contamination in nucleic acid purification, and QIAGEN's column design is optimized to limit carryover of contaminants. Although QIAquick 96 provides a high throughput solution, the elution, washing and binding steps are very laborious and time consuming if performed manually. With VIAFLO 96 handheld electronic pipette, the hands-on time is reduced, as samples and reagents can be transferred to all 96 wells at once. This enables rapid and efficient, high throughput PCR clean-up.
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