Fields & Applications Mass Spectrometry, Proteomics, Translational Science

Protein quantification at sub-nanogram loads using Zeno SWATH data-independent analysis (DIA) and nanoflow chromatography

Using DIA-NN software and the ZenoTOF 7600 system

contributed by SCIEX | 06/09/2022

The combination of the ZenoTOF 7600 system with nanoflow chromatography enables reproducible protein and peptide identifications using very low amounts of sample. Here, a 300 nL/min flow rate was used with a 45-minute gradient to identify hundreds to thousands of proteins at loads of 5ng and below. Loads down to 250 pg on column were investigated to simulate protein amounts obtained when analyzing low numbers of cells. This workflow highlights the high sensitivity of the ZenoTOF 7600 system for Zeno SWATH DIA proteomics workflows.

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE!

Email*

Choose a password

I have read and understand the Privacy Notice *

Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

Receive communication from The Cannabis Scientist I want to stay up to date with the Mass Spectrometry field I want to stay up to date with the Spectroscopy field

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.