Protein quantification at sub-nanogram loads using Zeno SWATH data-independent analysis (DIA) and nanoflow chromatography

The combination of the ZenoTOF 7600 system with nanoflow chromatography enables reproducible protein and peptide identifications using very low amounts of sample. Here, a 300 nL/min flow rate was used with a 45-minute gradient to identify hundreds to thousands of proteins at loads of 5ng and below. Loads down to 250 pg on column were investigated to simulate protein amounts obtained when analyzing low numbers of cells. This workflow highlights the high sensitivity of the ZenoTOF 7600 system for Zeno SWATH DIA proteomics workflows.