Ion exchange (IEX) is widely used for the analysis and purification of bio-molecules such as proteins, peptides and monoclonal antibodies.
In industrial-scale production, IEX is used for initial capture, intermediate purifications or final product polishing.
Challenges during mAb purification
For purification of monoclonal antibodies, high demands are required from the separating material. Factors influencing the binding characteristics of IgG are pH, linear velocity and/or salt concentration (conductivity) at the time the sample is loaded onto the column. Therefore, a material with highly stable performance is required with regard to all those factors. In order to demonstrate the performance of YMC-BioPro materials, several studies have been performed.
- Purification of Adalimumab
- Influence of pH
- Influence of linear velocity
- Influence salt concentration
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