Rapid and Accurate LC-MS/MS Analysis of Nicotine and Related Compounds in Urine Using Raptor™ Biphenyl LC Columns and MS-Friendly Mobile Phases

contributed by Restek | 09/16/2015

Clinical, Forensic & Toxicology Applications

A rapid, accurate, and reproducible method was developed for high-throughput testing of nicotine, cotinine, trans-3'-hydroxycotinine, nornicotine, norcotinine, and anabasine in urine. Data show that a fast and highly efficient analysis of these basic compounds can be achieved with the Raptor™ Biphenyl column using standard low-pH, reversed-phase LC-MS mobile phases that are compatible with a variety of LC-MS instrumentation.

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