Separation of oligonucleotides modified with disulfides using YMC-Triart Bio C4

In this application note the less hydrophobic wide-pore column YMC-Triart Bio C4 is used for the analysis of theses modified oligonucleotides due to the hydrophobic character of the disulfide units.

contributed by YMC | 03/18/2020

Antisense DNA and siRNA are widely used for gene silencing in research and medical applications. An effective delivery of the oligonucleotides into cells is important for clinical applications. As oligonucleotides are negatively charged the efficiency of the cell membrane permeability is low. Previous methods took several hours to deliver oligonucleotides to cytoplasm. Oligonucleotides modified with low molecular weight disulfide groups at their terminal residues reached the cytoplasm in 10 minutes as a result of disulfide exchange reactions with the thiol groups on cell surface.¹⁾

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