Introduction
A highly sensitive, linear and rugged extraction procedure for Catecholamines and Metanephrines from human plasma is presented. Extraction was performed using EVOLUTE® EXPRESS WCX 10 mg 96 well plates and was shown to give good recoveries and precision with the quick Load-Wash-Elute protocol.
Catecholamines and metanephrines are biomarkers used for the detection of diseases such as: hypertension, pheochromocytoma and neuroblastoma. The main target analytes are dopamine, epinephrine, norepinephrine, metanephrine, normetanephrine and 3-methoxytyramine (see Figure 1. for details). Levels are traditionally analyzed using liquid chromatography with electrochemical detection.
Figure 1. Structures of dopamine, epinephrine and norepinephrine (top), metanephrine, normetanephrine and 3-methoxytyramine (bottom).>> Download the full Application Note as PDF
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