Seamless Process from Protein Purification to Evaluation
This article describes a seamless process of target protein purification by affinity chromatography, fractionation, and re-injection for SEC using an inert HPLC system equipped with a liquid handler.
contributed by Shimadzu |
Size exclusion chromatography (SEC) is one technique used to separate molecules based on size, and it is one of the main methods used for verification of protein multimer formation. However, this technique can not distinguish proteins from ones with similar molecular sizes. Because smaller molecules can enter pores of materials packed in an SEC column, but larger molecules cannot. Therefore, larger molecules elute earlier than smaller molecules. In the case of target proteins in serum or culture supernatant, the samples have to be purified before SEC analysis. This article describes a seamless process of target proteins for purification, fractionation, and re-injection utilizing a liquid handler.
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