Cookies

Like most websites The Analytical Scientist uses cookies. In order to deliver a personalized, responsive service and to improve the site, we remember and store information about how you use it. Learn more.
Subscribe to Newsletter

Seamless Process from Protein Purification to Evaluation

Introduction

Size exclusion chromatography (SEC) is one technique used to separate molecules based on size, and it is one of the main methods used for verification of protein multimer formation. However, this technique can not distinguish proteins from ones with similar molecular sizes. Because smaller molecules can enter pores of materials packed in an SEC column, but larger molecules cannot. Therefore, larger molecules elute earlier than smaller molecules. In the case of target proteins in serum or culture supernatant, the samples have to be purified before SEC analysis. This article describes a seamless process of target proteins for purification, fractionation, and re-injection utilizing a liquid handler.

Read the full article now

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE!

Login
Receive content, products, events as well as relevant industry updates from The Analytical Scientist and its sponsors.
Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:
  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine

Register