In many laboratories, the need to consistently generate high-quality data means that laboratory managers and technicians need to ensure their instruments are performing optimally around the clock. Filtering samples before injection into a chromatography instrument is one of the primary ways that an analyst can protect their column and instrument from unnecessary wear and excess downtime. Filtration of both the sample and mobile phase prior to analysis helps increase the lifespan of chromatography columns reducing overall instrument wear and removing any particles that may interfere with the chromatogram. Sample filtration is most often performed using syringe filters as it is time effective and easy to implement method.
In the Analytical Technical Guide, we have shown that particulate removal through filtration can extend column life up to at least 52 times (Figure 2) over unfiltered samples. In addition to extending the life of the column, particulate removal also protects the pump, injector, and other components from premature wear.
Accurate, reproducible data depends upon proper HPLC column performance. Column blockages are prevented by filtering the mobile phase through a 0.2 µm or 0.45 µm disc filter, filtering samples through a 0.2 µm or 0.45 µm Pall Acrodisc One syringe filter, and utilizing inline filtration within the instrument. Without filtration, particles present in the sample can cause higher system pressures, shifted retention times, poor peak shape and separation.
Figure 2. Effects of Filters on HPLC Column Life
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