UHPLC analysis of cyclic peptides under LC/MS compatible conditions

4 antibiotic/immunosuppressing cyclic peptides are analysed using a YMC-Triart Bio C4 column, due to their hydrophobicity. Sharp peaks are obtained using a LC/MS compatible mobile phase at 70°C

contributed by YMC | 02/01/2022

Cyclic peptides are characterised by polypeptide chains that are arranged in a cyclic ring structure. This structure offers various advantages over their linear counterparts: on the one hand cyclic peptides show a higher thermal stability and a higher resistance to digestion. On the other hand their rigid structure results in a better biological activity since the binding to the target molecule is improved. All these characteristics turn them into highly valuable drug candidates.

Cyclic peptides have various applications; the peptides in this application note, polymyxin B sulfate, daptomycin and bacitracin are used as antibiotics for the treatment of bacterial infections, whereas cyclosporin A is used as an immunosuppressive agent.

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