(U)HPLC analysis of siRNA under denaturing and non-denaturing conditions using IP-RP and AEX

contributed by YMC | 07/05/2023

Small interfering RNA (siRNA) are short oligonucleotides, which effectively reduce gene expression. siRNAs can be analysed by high-performance liquid chromatography (HPLC) as double-stranded duplex under non-denaturing conditions or as two single strands under denaturing conditions. Examination of the siRNA under non-denaturing conditions can be helpful to prove the success of duplex formation and detect the amount of excess single stranded RNA.

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