UHPLC-MS analysis of phospholipids using a bioinert coated YMC-Accura Triart C18 column

Phospholipids are a key component of cell membranes. Commercially produced phospholipids can be applied e.g., in nanotechnology. The phosphate group which is contained in the hydrophilic head of the lipid poses a major challenge for chromatographic analyses. Phosphate containing compounds tend to be adsorbed on metallic surfaces such as the column body, frits, and tubing made from stainless steel due to ionic interactions with the electron-rich phosphate group. This effect is enhanced when working at low to neutral pH as metals become more electropositive. To overcome these challenges bioinert systems and columns such as the recently introduced YMC-Accura Triart columns are beneficial. YMC-Accura Triart columns have a bioinert coating on all surfaces, including the frits, to prevent any unwanted ionic interactions.