UHPLC/MS Analysis of Glyphosate and AMPA According to DIN ISO 16308

Water samples are derivatised according to DIN ISO 16308. The analysis of the resulting FMOC-derivatised glyphosate and AMPA is performed using a YMC-Triart C18 UHPLC column at an elevated pH of 9.5.

contributed by YMC | 02/15/2022

Glyphosate is the world’s most used herbicide. Since the 1970s it has been used to eliminate weeds in a non-selective way which means that all non-resistant plants are killed by its use. This resulted in the cultivation of genet-ically modified plants in order to make them glyphosate-re-sistant. Aminomethylphosphonic acid (AMPA) is the main metabolite of glyphosate and is found more often in the en-vironment than glyphosate. It is not only released into the environment by the metabolism of glyphosate, but also by the degradation of other urban phosphonates which is why higher concentrations in the environment are commonly known.

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