Ultra-Sensitive Method for the Determination of 1,25 di-OH Vitamin D2 and 1,25 di-OH Vitamin D3 in Serum Using Supported Liquid Extraction Prior to LC-MS/MS

contributed by Biotage | 03/16/2016

This application note describes a protocol for the extraction of 1,25 di-OH Vitamin D2 and 1,25 di-OH Vitamin D3 metabolites from serum using supported liquid extraction prior to LC-MS/MS detection. The method described in this application achieves highly reproducible recoveries for 1,25 diOH Vitamins D2 and D3. A calibration range between 5 and 500 pg/mL is demonstrated using a starting volume of 0.25 mL of serum. Sensitivity is maximized through the use of a simple PTAD derivatization and formation of a methylamine complex.

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