Advancing Biopharma Analytics: Insights and Innovations in Biomolecule Characterization
Discover the latest in analytical techniques with our curated resources on UV-Vis spectroscopy, biomolecule stability, and ID testing.
Designed to keep you updated on the newest developments in analytical methods, our collection includes whitepapers, application notes, technical overviews, interviews, podcasts, and videos, covering diverse subjects such as UV-Vis spectroscopy, biomolecule thermal stability, and novel identification testing strategies. Whether your focus is on drug development, quality assurance, or biochemical research, these resources provide essential insights and practical advice to enhance your work.
Characterization of Biomolecules using High-Performance UV-Vis Spectrophotometry
Characterization of biomolecules such as oligonucleotides and proteins is critical to better understanding biochemical and molecular processes. UV-Vis spectroscopy is widely used for stability studies, protein aggregation, DNA quantification and purity, and enzyme activity. The Cary 3500 UV-Vis offers advanced features to provide accurate and reproducible results.
DownloadOptimization of Thermal Stability Measurements for the Identification of Oligonucleotide APIs
All starting materials, including APIs, must undergo analytical identity (ID) determination before pharmaceutical manufacturing to ensure drug safety. Oligonucleotides pose challenges for ID due to sequence specificity. Melting temperatures (Tm) combined with intact mass measurements offer a solution using UV-based spectrometry for precise Tm determination at Vetter Pharma.
DownloadFast Determination of Thermal Melt Temperature of Double-Stranded Nucleic Acids by UV-Vis Spectroscopy
Double-stranded nucleic acid identity can be confirmed by measuring the thermal melt temperature (Tm) using UV-Vis spectroscopy. These Tm measurements can be time-consuming. However, the Cary 3500 UV-Vis systems can measure Tm quickly without sacrificing the quality of the results. The study demonstrates that Tm values are consistent across various ramp rates, ensuring reliable and reproducible results for laboratory applications.
DownloadBest Practice for Nucleic Acid Thermal Stability Measurements Using the Cary 3500 UV-Vis Spectrophotometer
The Cary 3500 UV-Vis systems use temperature-controlled UV-Vis spectroscopy to analyze molecules' thermal stability. They are essential for nucleic acid quantification quality control by measuring absorbance at 260 nm and performing thermal melt identification verification. Melting temperature (Tm) analysis is key for studying nucleic acid denaturation.
DownloadCharacterization of Protein Thermal Stability Using UV-Vis Spectroscopy
The drug discovery process involves identifying and characterizing drug targets to find lead compounds. Biochemical characterization (e.g., molecular weight, solubility, thermal stability) is crucial for protein therapeutics. Thermal stability studies measuring melting temperatures help understand protein stability, ligand-binding, and optimal storage, aiding drug development and commercialization.
DownloadEvaluating an Innovative Analytical ID Testing Strategy for Oligonucleotides
Vetter Pharma successfully uses the Cary 3500 UV-Vis to optimize thermal melt measurements of oligonucleotide-based pharmaceuticals.
DownloadCharacterization of Antibody-Drug Conjugate CQAs Using the Agilent Cary 3500 UV-Vis
Antibody-drug conjugates (ADCs) are biotherapeutics that require monitoring of critical quality attributes (CQAs) like drug-to-antibody ratio (DAR) and aggregation. The Agilent Cary 3500 UV-Vis Multicell spectrophotometer efficiently analyzes DAR and aggregation index at multiple temperatures simultaneously, offering simple methodology, accurate temperature control, and confidence in results.
DownloadExperts Evaluate an Innovative Analytical ID Testing Strategy for Oligonucleotides
In this podcast, Dr. Melanie Zerulla-Wernitz, Head of the Analytical Science Laboratory at Vetter Pharma, discusses the tools and techniques her team uses to optimize analytics of oligonucleotide-based pharmaceuticals. Dr. Mathieu Rault of Agilent Technologies also highlights how to execute temperature-controlled measurements of oligonucleotides and outlines the implementation of spectroscopy tools for fast, accurate ID testing.
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