Oligonucleotide Analysis

Robust, accurate characterization of your oligonucleotide therapeutic

You have likely started to hear about oligonucleotides and mRNA more frequently than in years past...and there is a good reason for that! Therapeutic oligonucleotides comprise a rapidly expanding category of drugs that will change the standard of care for many diseases and actualize personalized medicine.

The rapid growth of this therapeutic area has been made possible by recent advancements that include:

  • Overcoming the previous challenges of controlled immune response

  • Improved product stability

  • Optimizing the delivery system to reach its therapeutic target

The analysis of oligonucleotide therapeutics requires robust and accurate analytical characterization to confirm identity, and to determine purity, quality, and strength. Below are several educational assets and resources highlighting Thermo Scientific optimized solutions for oligonucleotide analysis.

VIDEOS

Why vaccine developers need a Charged Aerosol Detector

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WEBINAR

Oligonucleotide analysis, new practical advances and tips to a mature technique

Analytical solutions for mRNA vaccines and therapeutics

Messenger RNA (mRNA) therapy enables the body to make the proteins we need to prevent, treat, or cure diseases. Unlike traditional biologics, mRNAs are large and delicate molecules that are produced using in vitro transcription (IVT), which need to be protected by lipid nanoparticles (LNPs) before they reach target cells. Analytical characterization of mRNA therapeutics presents unique challenges that require new technologies and solutions.

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DNAPac columns for superior oligonucleotide analysis

Explore our selection of Thermo Scientific DNAPac columns and discover how they facilitate high resolution HPLC and UHPLC separations of oligonucleotides, enabling reliable oligonucleotide analyses that meet the throughput demands of your laboratory.

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Characterization of mRNA 5’ capping products using an LC-HRAM-MS/MS analytical platform and Thermo Scientific BioPharma Finder software solution

Develop a sensitive and robust LC-HRAM-MS/MS method for identification, sequence confirmation, and relative quantitation of the 5’ cap in in vitro transcription (IVT) mRNA products.

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Identification and quantitation of oligonucleotides, impurities, and degradation products

The capabilities of high-resolution accurate mass (HRAM) and data-dependent tandem mass spectrometry (ddMS2) enables confident identification, mapping, and relative quantitation of oligonucleotides, impurities, and degradation products in a single experiment.

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High resolution LC/MS analysis of therapeutic oligonucleotides

LC-HRAM is indispensable for determining impurity levels. As several modified full-length impurities, the n − 1 deletion and n + 1 addition sequences often cannot be baseline separated chromatographically and it is therefore challenging to quantitate at low levels.

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Oligonucleotide mass confirmation

Expanding a UV quality control method with MS provides mass confirmation and impurity identification.

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Develop and optimize a liquid chromatography (LC) ddMS2 method for the untargeted analysis of oligonucleotides using Orbitrap-based HRAM MS, describing oligonucleotide identification and mapping using the Oligonucleotide Analysis workflow available in BioPharma Finder software.

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Characterization of a synthetic double stranded siRNA using high-resolution mass spectrometry

Demonstrate the capability of separating and characterizing a double-stranded siRNA and its diastereomers using a Thermo Scientific™ Vanquish™ Horizon UHPLC system coupled with a Thermo Scientific™ DNAPac™ RP column, a Thermo Scientific™ Orbitrap Exploris™ 120 mass spectrometer, and Thermo Scientific™ BioPharma Finder™ software.

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