Oligonucleotide Analysis
Robust, accurate characterization of your RNA-based therapeutics.
RNA therapeutics comprise a rapidly expanding category of drugs that will change the standard of care for many diseases and actualize personalized medicine. These drugs are cost effective, relatively simple to manufacture, and can target previously undruggable pathways.
The rapid growth of this therapeutic area has been made possible by recent advancements that include:
Overcoming the previous challenges of controlled immune response
Improved product stability
Optimizing the delivery system to reach its therapeutic target
The analysis of RNA-based therapeutics requires robust and accurate analytical characterization to confirm identity, and to determine purity, quality, and strength. Below are several educational assets and resources from Thermo Fisher Scientific highlighting optimized solutions for RNA-based therapeutic analysis.
This application note demonstrates impurity identification and sequence mapping using ddMS2 data collected on the Thermo Scientific™ Orbitrap Exploris™ 240 mass spectrometer.
More information >Characterization of a synthetic double stranded siRNA using high-resolution mass spectrometry
In this application brief, we extend the application of the ddMS2 method to characterization of a synthetic double-stranded siRNA consisting of complementary sense and antisense oligonucleotides.
More information >Analyzing oligonucleotides? Check out our webinar series!
Join us to learn from experts in the field about major advancements, optimizations, and innovations of analytical technology to take your oligonucleotide analysis to the next level. Through our external and internal expert speakers’ testimonials, you will have the chance to discover complete solutions and get a full understanding of new advancements and optimization techniques for each step of the workflow.
Watch on demandOligonucleotide mapping using Thermo Scientific™ BioPharma Finder™ software
Fast, accurate mass measurement and confident mapping of oligonucleotides.
More information >Analytical solutions for mRNA vaccines and therapeutics
Unlike traditional biologics, mRNAs are large and delicate molecules that are produced using in vitro transcription (IVT), which need to be protected by lipid nanoparticles (LNPs) before they reach target cells. Analytical characterization of mRNA therapeutics presents unique challenges that require new technologies and solutions.
More information >Characterization of mRNA 5’ capping products using an LC-HRAM-MS/MS analytical platform and Thermo Scientific™ BioPharma Finder™ software solution
A sensitive and robust LC-HRAM-MS/MS method for identification, sequence confirmation, and relative quantitation of the 5’ cap in in vitro transcription (IVT) mRNA products.
More information >Characterization of in vitro-transcribed (IVT) mRNA poly(A) tail by LC-HRAM-MS and BioPharma Finder™ 5.0 software
A sensitive and robust LC-MS method for identification and sequence confirmation of 3' polyadenylated tails from IVT mRNA products.
More information >mRNA direct sequence mapping using automated partial digestion with magnetic nuclease and LC-HRMS
In this study, a workflow will be presented for direct sequence analysis of mRNA using UHPLC and HRMS fragmentation.
More information >