Revolutionizing Chromatography: Insights into Axcend Focus LC® for Lab Efficiency
Axcend’s eco-friendly, compact and portable HPLC instruments and accessories are engineered to deliver consistent, high-quality performance, ensuring precise and dependable analytical results.
The trend towards miniaturization in lab instruments is transforming the landscape of scientific research and diagnostics. Advances in high-performance liquid chromatography (HPLC) technology have allowed smaller instruments to achieve high levels of sensitivity and precision, often matching or exceeding their larger counterparts.
In biopharma analysis, where precision and sensitivity are crucial, smaller lab instruments help streamline processes, reduce costs, and enhance the reliability of results. The ability to conduct comprehensive analyses with minimal sample sizes is a game-changer, especially when dealing with expensive or scarce biologics.
The Axcend Focus LC is a compact capillary liquid chromatograph that offers increased productivity, ease of integration and method transfer, and an environmentally friendly option for HPLC analyses. The Focus LC is small enough to be easily placed near the sample source including under the hood, yet powerful enough to be used in any state-of-the-art laboratory. Couple the Focus LC with InFocus™ for reaction monitoring and dissolution testing or with the FocusArray™ for full spectrum detection in development. The Focus LC is easily integrated with automated systems and digital platforms, enhancing data collection, analysis and remote monitoring.
The award-winning design of the Axcend Focus LC includes low-volume interfacing kits for connections to virtually any mass spectrometer with an appropriate source and enables improved sensitivity for LCMS and LCMS/MS applications.
This collection of resources and application notes explore capillary HPLC systems and the diverse range of applications for compact LC to almost any application that is at-line, online, or truly portable.
InFocus™
Engineered to provide real-time information that accelerates the discovery and development process, the Axcend system is the only separations Process Analytical Technology (PAT) system that’s small enough to be placed in a hood or immediately adjacent to the reaction vessel. The solution consists of the Axcend Focus LC, InFocus™, and Axcend cartridge containing the column oven and UV detector.
DownloadDetermination of Pharmaceuticals by Capillary HPLC-MS/MS
The widespread use of pharmaceuticals including hormones, antibiotics, non-steroidal anti-inflammatory drugs, antidepressants, and antifungal agents results in significant discharge of these drugs and their metabolites into wastewater. Concerns have been raised over their potential to contaminate ground and surface waters, and to enter drinking water supplies. Therefore, monitoring pharmaceuticals in aqueous samples represents an important analytical task. Using capillary LC for this purpose provides many advantages including dramatically reduced consumption of toxic and expensive organic solvents.
DownloadThe Axcend Focus LC Spec Sheet
Introducing the Axcend Focus LC:
Portability: Weighs only 7.82 kg and includes a convenient carrying handle and battery for remote operation.
Eco-Friendly: Uses 1000X less solvents and produces minimal waste, making it a leader in green analytical chemistry.
Versatility: Ideal for applications in pharmaceuticals, chemicals/petrochemicals, education, food & beverage, and more.
Advanced Technology: Easily coupled with other instruments, such as mass spectrometry.
DownloadHow Green is Your HPLC?
The Focus LC sips a miniscule volume of reagents and samples, significantly reducing solvent costs and waste while preserving precious sample volumes. Whereas a single legacy HPLC can fill a 10 L waste container in about 2 weeks; it would take 40 years for the Focus LC to fill the same. Energy consumption is also a fraction of legacy LCs. Using the Green Chemistry Institute’s Greenness Score, the Focus LC is about 180 times greener than legacy LC systems.
DownloadTargeted Peptide Quantification with Small Footprint Capillary LC-MS/MS
Quantification of low-abundance proteins remains a challenge for proteomics research. Standard LC-MS/MS often lacks the sensitivity to detect the crucial players in pathways, cellular processes, or biopharma manufacturing. Capillary LC-MS/MS emerges as a compelling solution, offering exceptional sensitivity and minimal sample volume requirements. This technique not only boasts superior detection limits but also dramatically reduces hazardous organic solvent consumption. These low flow rates also synergistically enhance sensitive mass spectrometry detection due to droplet size reduction. We present a solid method for peptide quantification using capillary LC-MS/MS, emphasizing three key analytical parameters: (1) limit of detection (LOD) and limit of quantification (LOQ), (2) linear range for multiple peptides, and (3) precision. Sensitivity comparison between capillary LC/MS and standard (std) LC/MS was also conducted. Sensitivity for all peptides surpassed standard-flow LC-MS significantly.
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