Working on Antibody-Drug Conjugates (ADCs)?
Antibodies targeting cell surface antigens, can trigger endocytosis leading to their internalization, a process known as antibody internalization (ABI). Monitoring ABI is vital in Antibody Drug Conjugate (ADC) design, as factors such as the target antigen's epitope, ADC-antigen affinity, and intracellular trafficking, affect internalization.
ADCs combine immunotherapy and chemotherapy for targeted cancer treatment, they represent one of several modifications made to monoclonal antibodies (mAbs) to enhance their anti-tumor activity.
Characterization of T Cell Activation and Function in Cancer Immunotherapy Using High-Throughput Cytometry
This application note demonstrates the value of using High-Throughput Screening Cytometry in conjunction with validated Human T Cell Activation Kits, to assess immune cell phenotype and function during the development of antibody-mediated and cell based cancer therapies using minimal sample volumes.
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iQue® 5 Truly Fast. Simply Effortless. High-Throughput Screening (HTS) by Cytometry, recognized for effortless operation to empower every user. Provides our most powerful screening capabilities for modern applications, complete with enhanced software features and simplified extended operation for maximum data generation. Continuous plate loading through integration with any automation system is available.
DownloadPhenotypic and Functional Characterization of CAR-T Cells with High-Throughput Screening by Cytometry and Live-Cell Analysis
Data examples shared in this whitepaper demonstrate how the use of both live-cell analysis and HTS by Cytometry can add value when developing and characterizing T cell therapies.
DownloadExploring Antibody Internalization: Case Studies Using the iQue® HTS Platform
Showcasing a versatile tool and methods in the study of antibody internalization (ABI), which offer enhanced capabilities for complex workflows. Through three different case studies, including the characterization of Antibody Drug Conjugates (ADCs) and monocyte differentiation, the technique's ability to measure ABI efficiently is demonstrated.
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