Three Gurus of Supercritical Fluid Chromatography
Is the ongoing resistance to SFC a simple misunderstanding and the legacy of past disappointments? Here, Isabelle François, Davy Guillarme, and Eric Lesellier highlight the positive aspects of this underused technique and urge sceptics to re-evaluate the potential of modern SFC systems.
Isabelle François, Davy Guillarme, Eric Lesellier |
What are the main advantages offered by SFC?
Isabelle François: SFC can be used in a wide range of industries. In laboratories where organic synthesis is carried out, the medium used for synthesis is very often an apolar solvent, which can be easily injected into an SFC system. The pharmaceutical industry is significantly benefitting in this respect from this technology. Achiral SFC provides an alternative method to RPLC to search for additional impurities present in the sample, whereas enantiomers are separated by chiral SFC. Chiral separations are the niche application for SFC. SFC is even more advantageous when separations are scaled up to preparative mode. Compared with normal-phase liquid chromatography (NPLC), preparative SFC uses less solvent, produces less waste, and requires less time for fraction evaporation. The result is a greener, more cost-effective solution.
In the food industry, SFC can be used for the analysis of nutrients, vitamins, lipids and so on. In nutriceutical applications, it can aid in the search for interesting compounds, certainly when coupled with time-of-flight mass spectrometry.
In the petrochemical industry, applying a pressure gradient allows the density of the mobile phase to be increased, resulting in enhanced solvent strength, which allows the implementation of flame-ionization detection (FID).
Read the full article now
Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE and always will be!
Or register now - it’s free and always will be!
You will benefit from:
- Unlimited access to ALL articles
- News, interviews & opinions from leading industry experts
- Receive print (and PDF) copies of The Analytical Scientist magazine
Or Login via Social Media
By clicking on any of the above social media links, you are agreeing to our Privacy Notice.