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The Analytical Scientist / Product Profiles / 2016 / HPLC affinity column for fast and accurate antibody titer analysis

HPLC affinity column for fast and accurate antibody titer analysis

HPLC affinity column for fast and accurate antibody titer analysis

In many stages of mAb development, harvest cell culture samples must be screened for their IgG titers. Antibody titer determination by Protein A affinity HPLC is much more robust, reliable and reproducible than enzyme-linked immunosorbent assays (ELISAs). TSKgel Protein A-5PW is specifically designed for the fast determination of mAb concentrations within 2 minutes analysis time. Its wide dynamic range allows equally accurate determination of comparatively low mAb titers in early development and high mAb titers of optimized cell lines in production. With the ability to perform more than 2000 injections per column this new affinity column provides a robust tool for mAb titer analysis.
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