Of the four common causes for HPLC column failure (plugging, voids, adsorbed sample, and chemical attack), plugging is the most frequently encountered by analytical chemists or analysts. Filtration is well documented as a maintenance tool for HPLC analyses.
Using a latex sphere solution, an unfiltered HPLC sample failed after 19 injections due to plugging of the column, reaching a backpressure of 3500 psi. Using Acrodisc® One syringe filters with a 0.45 μm membrane, no increase in backpressure was observed after 1000 injections.
To read the full test, visit our website and download the Acrodisc One technical guide
