Static Light Scattering Technologies for GPC/SEC Explained
The aim of this guide is to provide the reader with a clear understanding of the different technological approaches used to measure molecular weight by static light scattering in a GPC/SEC experiment.
sponsored by Malvern Panalytical
It will explain and differentiate between the techniques and technologies used in SLS, MALS, RALS, and LALS. It assumes no prior knowledge of the light scattering theory or instrumentation and should be ideal for those new to light scattering and those looking to increase their knowledge in the area. The guide covers an introduction to the theory and background of molecular weight measurements by static light scattering. It is hoped that the information contained in here will help users to make an informed decision about the most appropriate light scattering technology to use.
SUMMARY
Static light scattering is a technique to measure the molecular weight using the relationship between the intensity of light scattered by a molecule and its molecular weight and size. These relationships are described by Rayleigh theory which states that the molecular weight of a molecule is proportional to the Rayleigh ratio of scattered light i.e. the ratio of scattered light intensity to incident light intensity.
All static light scattering instruments detect the amount of light scattered by a sample to measure its molecular weight; however, as molecules grow in size, a second factor called angular dependence becomes significant. Angular dependence affects the intensity of scattered light and hence the calculated molecular weight. It must therefore be accounted for.
