Conexiant
Login
  • The Analytical Scientist
  • The Cannabis Scientist
  • The Medicine Maker
  • The Ophthalmologist
  • The Pathologist
  • The Traditional Scientist
The Analytical Scientist
  • Explore

    Explore

    • Latest
    • News & Research
    • Trends & Challenges
    • Keynote Interviews
    • Opinion & Personal Narratives
    • Product Profiles
    • App Notes

    Featured Topics

    • Mass Spectrometry
    • Chromatography
    • Spectroscopy

    Issues

    • Latest Issue
    • Archive
  • Topics

    Techniques & Tools

    • Mass Spectrometry
    • Chromatography
    • Spectroscopy
    • Microscopy
    • Sensors
    • Data and AI

    • View All Topics

    Applications & Fields

    • Clinical
    • Environmental
    • Food, Beverage & Agriculture
    • Pharma and Biopharma
    • Omics
    • Forensics
  • People & Profiles

    People & Profiles

    • Power List
    • Voices in the Community
    • Sitting Down With
    • Authors & Contributors
  • Business & Education

    Business & Education

    • Innovation
    • Business & Entrepreneurship
    • Career Pathways
  • Events
    • Live Events
    • Webinars
  • Multimedia
    • Video
Subscribe
Subscribe

False

The Analytical Scientist / App Notes / 2017 / A new era in proteomics: spectral library free data independent acquisition (directDIA)

A new era in proteomics: spectral library free data independent acquisition (directDIA)

05/18/2017

Share

Featured Image
Introduction

Discovery proteomics aims to understand global proteome dynamics for instance in cells, tissue or an organism. The key to gain a comprehensive picture of the biology lies in the quantitative precision, reproducibility and the unbiased nature of analysis at the highest possible protein coverage.

Modern discovery proteomics workflows mainly rely on high-resolution LC-MS/MS instruments. Until today data dependent acquisition based proteomics (DDA) is the most widely used discovery proteomics technique. A major limitation of DDA based proteomics however, is the semi-stochastic peptide selection for its identification. Therefore, the same peptides will not be identified reproducibly even when analyzing technical replicates. Despite the possibility of performing MS1 alignment, this results in high number of missing values in the data matrix that lead to problems in statistical analysis and an incomplete biological picture.

Recently, data independent acquisition methods (DIA) have emerged as an alternative where in a single measurement all detectable peptides can be quantified with high sensitivity, quantitative precision and reproducibility. However, for best performance, a spectral library typically generated from DDA runs is necessary for targeted data analysis of DIA. This increases the instrument run time and especially in small experiments presents a significant cost overhead. Biognosys, the leading developer of nextgeneration proteomics solutions, is now adding a new workflow called directDIA to its Spectronaut software. DirectDIA enables reproducible and precise quantification of thousands of proteins in a single sample without the need for DDA based spectral libraries. This is a simple workflow for label-free proteome quantification that offers significant savings in instrument time while maintaining high quantitative precision and high reproducibility at the same level as the targeted analysis of DIA data using spectral libraries. In this technical note, we present an overview of the directDIA workflow and its application to a set of six human liver samples derived from healthy and adjacent cancerous tissue. In this data set, we quantified over 70,000 peptide precursors corresponding to over 5,300 protein groups. The quantitative data completeness is >90% for peptide precursors.If you have any questions about the technical note or Biognosys’ discovery proteomics platform please contact us at [email protected].

>> Download the full Application Note as PDF

Newsletters

Receive the latest analytical scientist news, personalities, education, and career development – weekly to your inbox.

Newsletter Signup Image

Explore More in Analytical Science

Dive deeper into the analytical science. Explore the latest articles, case studies, expert insights, and groundbreaking research.

False

Advertisement

Recommended

False

Related Content

Solvents & Inorganics for accurate, brilliant results
Solvents & Inorganics for accurate, brilliant results

January 9, 2017

Now that the life science business of Merck KGaA, ...

Discovery of Sulfur-Containing Compounds in Broccoli with GC-TOFMS
Discovery of Sulfur-Containing Compounds in Broccoli with GC-TOFMS

January 16, 2017

Analyzing samples of a raw broccoli and a broccoli that was processed to be sold frozen we show the sample-distinguishing differences that occur during food processing...

Aquastar® reagents for brilliant Karl Fischer titration results
Aquastar® reagents for brilliant Karl Fischer titration results

January 23, 2017

Determining the water content of gases, liquids and solids can be achieved with a high degree of accuracy using Karl Fischer titration together with our Aquastar® reagents and standards from Merck...

REACH Polymer Status determined with GPC/SEC
REACH Polymer Status determined with GPC/SEC

January 27, 2017

Polymers are “special substances” in terms of REACH...

False

The Analytical Scientist
Subscribe

About

  • About Us
  • Work at Conexiant Europe
  • Terms and Conditions
  • Privacy Policy
  • Advertise With Us
  • Contact Us

Copyright © 2025 Texere Publishing Limited (trading as Conexiant), with registered number 08113419 whose registered office is at Booths No. 1, Booths Park, Chelford Road, Knutsford, England, WA16 8GS.