Abstract
In this report, the complete automation of dried blood spot analysis is demonstrated and the results evaluated. A novel, automated DBS Autosampler (DBS A) automatically inserts DBS cards into a Flow Through Desorption (FTDTM) cell in which individual blood spots are rapidly and effectively desorbed. The DBS A is integrated into a complete cleanup and analysis system using online SPE with replaceable cartridges combined with automated injection to an LC/MS/ MS system. Automated DBS extraction methods for a variety of analytes from different matrices are examined along with the use of different SPE cartridge sorbents. The resulting precision and accuracy data are provided.
Introduction
Dried blood spot sampling is gaining interest in the scientific community as an alternative technique for acquiring and storing the necessary blood samples for analysis. In addition to the ease of sampling afforded by this technique, DBS also translates into fewer animals used during pre-clinical studies and better quality of data during studies since serial sampling from one animal is possible. The dried samples are easily shipped and enable storage at room temperature, which provides additional cost savings and simplified logistics. Furthermore, samples can be collected in remote locations and shipped to the laboratory without conservation or refrigeration.After receiving a DBS card in the lab, the time consuming manual sample preparation steps such as spot punching, elution, and centrifugation can be eliminated using the GERSTEL DBSA system shown in Figure 1. Sample desorption from the dried blood spot is performed in a highly reproducible manner and the defi ned area of the matrix spot is completely desorbed by means of the patented Flow-Through Desorption (FTDTM) technology as shown in Figure 2.
Figure 1. DBSA-SPExos-LC/MS/MS configuration.
Figure 2. Flow-through desorption technology.>> Download the full Application Note as PDF
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