Summary
Simulated moving bed chromatography (SMBC) was applied for the purification of xylitol from fermentation mash of a fed batch culture. This process enabled to purify xylitol with nearly 100 % purity and recovery. Thus, allowing large scale purification of xylitol from biological xylose-xylitol conversion process.
Introduction
Within the European Valor Plus research project an alternative, biological way of xylose conversion was investigated. By using a Candida yeast strain, xylose from a hemicellulose hydrolysate was converted to xylitol. HPLC analysis of the fermentation mash revealed that the xylose to xylitol conversion was successful. Previous batch HPLC experiments (App. note VFD0155) indicated the potential to apply SMBC for this purification task. The separation was performed in isocratic mode on polymer based Eurokat columns and the target substance xylitol eluted at the end of the chromatogram, all factors enabling a SMB process. SMB chromatography is a continuous chromatography technique that separates binary or pseudo-binary mixtures into pure substances or fractions. Compared to traditional batch chromatography this process leads to higher yields of purified substances while consuming less eluent and packing material.
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