Instrumentation & Experiment
The Confocal Raman Microscope alpha300 R combines a highly sensitive confocal microscope and a high-transmission Raman spectroscopy system. With a resolution down to 200 nm at each image pixel, a complete spectrum is typically acquired in only 10 to 100 ms. The number of image points (spectra) is limited only by the computer memory. A typical image consists of 10,000 (100 x 100) to 65 536 (256 x 256) spectra. An image is generated from this multi-spectrum file by integrating over a specific Raman line or a region in all spectra. As all spectra are stored in memory, a variety of properties such as peak-width, center of mass or peak position of certain Raman lines can be calculated from a single measurement. For depth profiling measurements, the focal plane can be moved in the z-direction when performing either xz scans or generating x-y image stacks in the z direction.

