Introduction
Powdered Decaffeinated Green Tea Extract is prepared from the young, unfermented leaf and leaf buds of Camellia sinensis (L.) Kuntze (Fam. Theaceae), also known as Thea sinensis L.
The proposed method has been submitted for consideration in the USP DSC 2018.
Scope This HPTLC method identifies Powdered Decaffeinated Green Tea Extract as defined by USP DSC 2018. Recommended devices Automatic TLC Sampler (ATS 4), Automatic Developing Chamber (ADC 2), Derivatizer, TLC Visualizer, visionCATS. Samples 50 mg/mL of Decaffeinated Green Tea Extract in methanol and water (7:3, v/v) sonicated for 10 min, and centrifuged. Use the clear supernatant. Standards Standard solution A: 0.5 mg/mL of USP chlorogenic acid RS, (-)-epigallo-catechin, and USP caffeine RS in methanol. Standard solution B: 50 mg/mL of USP Powdered Decaffeinated Green Tea Extract RS in 70% methanol.
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