Measurement of the internal volume of an LC column is useful for a number of aspects of liquid chromatography, including determination of appropriate equilibration times, gradient reequilibration times, setting appropriate injection volumes and performing gradient adjustments and translations. This Knowledge Note explains how the column volume can be calculated and experimentally measured.
Estimating Column Volume (VM)
A convenient estimate of VM in mL, can be obtained using the following equation.
where L is the column length in mm and dc is the column internal diameter in mm. This method provides an estimate accurate to within approximately 10% and is suitable for applications where an accurate measurement of VM is not essential, e.g. establishing appropriate equilibration times or postgradient re-equilibration times for a column.
>> Download the full Application Note as PDF
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